FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_sp29_6_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_sp29_6_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144342
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2700.18705574261129815No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAACA150.002352345768.9927144
GTATTAA200.007339051551.7624861
CTAGCCA200.007339051551.7624869
GCGCGGG306.285534E-446.01119
CACTCCT450.00462749230.6421866
AGCGCAG500.007703791427.6066610
ACCTGGC500.00771684927.5970862
CAACACC500.00771684927.5970864
GAGACTT700.001329018524.6402575
TGTACAA800.00288905621.5602232
GTGTTGA1004.3025133E-420.7049941
ATAAAAC900.005714125519.1646443
TGTGTAG1301.17916585E-418.56209269
TGCTCGC1452.6967772E-416.65919110
AATAAAA1554.4936515E-415.5790012
CTGGATA1350.00321952815.33703320
TTGCTCG1657.203984E-414.63989459
AGAACTG1657.222963E-414.6348195
TCTGGAG1450.005189301414.2743563
GTGTGTA1709.102607E-414.1945468
Produced by FastQC (version 0.11.2)