FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_sp29_5_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_sp29_5_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29622
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG640.21605563432583888No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAA150.00233288669.01
GTTGAGG200.00728878951.759
GTTTATT200.00728878951.7539
ACTACAT200.00728878951.7544
TCGTACT200.00728878951.752
TGTTCCA350.001326674239.42857466
TTCAAAA500.00761578427.612
ACGACTC1653.6420453E-516.72727267
GACTCCT1704.7040547E-516.23529469
CGACTCC1807.669575E-515.33333368
GACGACT1807.669575E-515.33333366
CGACGAC2052.3191655E-413.46341465
CCGACGA2255.093122E-412.26666664
GCCGACG2550.001455172810.82352963
TGCCGAC2800.00316776949.85714362
CGCTGCC3501.7621873E-49.85714269
ACGCTGC3703.0831524E-49.32432568
CTGCCGA3050.0064169019.0491861
GCTGCCG3100.0073340388.90322660
GACGCTG4200.00109202318.21428567
Produced by FastQC (version 0.11.2)