FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_sp29_4_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_sp29_4_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168565
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3540.21000800877999584No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTC501.8538468E-434.5026747
ATATTAG400.00259664234.4821782
GCATAAA400.002604218434.4617081
GGGATGG450.00459645730.6872810
CGCCGTA605.4569804E-428.70105267
TGACACG500.00770237227.61034240
AATAGAG900.005711965319.1681525
GTGTGTC950.00780392418.1593027
ACTCTGT3900.016.77907869
TACTCTG3900.016.77907868
CTGTGTA1450.00524950214.25155769
AAATAAA1500.006444022513.81749326
AGTGGTG1850.001713763313.0550678
CTACTCT4508.731149E-1113.01114467
TCTGTGT2457.770573E-512.65189368
CTGATAT2100.004422238611.5043119
ATCTACT6351.8189894E-1211.39002369
GTGGTGA2450.001083508311.2695289
TAGGCTT4004.88948E-711.22337517
AGGCTTA4004.88948E-711.22337518
Produced by FastQC (version 0.11.2)