FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_sp29_40_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_sp29_40_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences330901
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6430.19431793799353886No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGGG252.5700065E-455.1760563
TCTAGGG350.001349302839.4114652
GCGGATG450.00461411630.6719111
AGTCTGC450.00462095130.6626328
TAGTCTA450.00462095130.6626327
CTATTAC450.00462437230.6579951
CGTGGGG500.007703542727.61725618
ACCGCAT500.007732040727.5963738
GAGCCTC700.001334035524.635895
TAGAACA953.0682245E-421.780024
GTCATAT850.00412081820.2883821
TCTTACA850.00412442820.2853132
CAACGTT900.00573031619.1641456
CTGAGCC900.00573031619.1641459
TCCAATA900.005740341319.158352
TCCTGTA950.00784256218.1500172
TTACACT950.00784256218.1500174
GTTCATA1200.001476562217.2451231
ACTGGAC1250.001942302616.5528143
TAAGCCA1704.9109698E-516.24790428
Produced by FastQC (version 0.11.2)