FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_sp29_3_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_sp29_3_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135708
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2940.21664161287470157No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTTAC200.007323882551.78790329
ATATAGT200.007355996451.7306561
TCTTGCA501.856879E-434.4871062
GCTCGCC400.002593057234.4871038
CTTCAGG605.409181E-428.739251
ACATGTA500.007711489727.5998533
AGACGGT500.0077253827.5896846
GTCTTGC500.0077253827.5896841
ATTGTTC850.004110672520.2865336
AGTGTAG1906.952556E-616.33599568
CGGAGGA1300.002512631715.9171254
GTGTAGA1958.897652E-615.91712469
TAGTGTA1958.897652E-615.91712467
ATAGTGT2253.441066E-513.79484266
TTTCTCC1800.001396450913.4116523
CTGGATA1550.00804287213.35969220
GCTTAGG2601.3331923E-411.9378438
CTTAGGC2802.6549934E-411.085149
AGGATAG3501.5721396E-510.83880469
GGATAGT2903.673914E-410.70289464
Produced by FastQC (version 0.11.2)