FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_sp29_39_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_sp29_39_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences418181
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7720.18460905684380685No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCAT809.576872E-525.8633356
CTAGACA902.1331581E-422.984134
CGTAATA800.00291204221.5424692
CATGCCG1258.7141794E-519.3228510
TCTAGAC1258.770439E-519.306673
GAGGTAG900.005751331319.1534421
ACAAGGG900.005751331319.1534423
ATGCCGT1108.203371E-418.82546411
GATACCA1108.256363E-418.8074475
TAAAGAG950.00785211918.1475375
CTAGACC950.00785753818.1453654
TACCATG1150.001109299717.9983447
CCTGCAC1250.001946791216.5485743
ACCATGC1756.346095E-515.76997958
TGATACC1554.5393055E-415.5698954
TCCGACT1400.004098035414.80029725
TACTCCT8950.014.24761769
AGGCATA1500.00649782213.80699430
CCTCTTA1500.006549545613.7904791
TGTGTAG2801.329503E-613.53936169
Produced by FastQC (version 0.11.2)