FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_sp29_38_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_sp29_38_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences373247
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7650.20495811084884807No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCATA200.00735182251.75593638
CCTAAGG350.001349442639.4119573
TATACTC451.0003796E-438.332595
TTACACC501.8606933E-434.49474
GGTTACA553.253214E-431.3630261
GTCTAAC450.004619167730.666071
AGGCCGC605.415884E-428.7494418
CCCTAAG500.0077442527.5883662
TTATACT658.65384E-426.5343884
GTATACA700.001332309624.6423781
TATTATA851.4448776E-424.3426782
CACGTGC750.001990738322.9995546
TATACAG750.001995399822.9903072
TACACCG800.00289612721.562085
TAGACTG850.00411551120.2937245
CTCCTAT850.00411551120.2937241
TATATAA850.00412510720.2855642
TACTACA900.00574127819.158592
GCATAGG950.00780753118.16484832
TAGACAG950.00782566618.1575435
Produced by FastQC (version 0.11.2)