FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_sp29_37_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_sp29_37_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences432935
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6730.15545058727060643No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTAGG400.002597111534.4930651
CTATAAG450.004624082730.66051
GTATAAC703.8508035E-529.5654811
GTCTAAG500.007737261727.5944481
GTATTAC809.561375E-525.8697991
TAACACC700.001336764324.6293564
TAAGAGT1001.5673719E-524.136774
GTATTGG1151.6965314E-623.9951741
TTACACT1301.8516221E-723.871534
CATACGC1052.2821067E-522.99271651
ATATGGG750.001997379422.98743
CTAAGAG902.131271E-422.98743
TACCCTT1103.2717413E-521.9450535
ACCCTTT800.00290381621.5531796
CTATACT800.002905764821.5506884
GCTTACC850.004107109720.30177511
CTAAGAC850.004109862720.29942745
ATATAAG1056.022814E-419.710321
GGTTTAC900.005735416419.1628111
ACGCATA1802.000179E-719.1428954
Produced by FastQC (version 0.11.2)