FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_sp29_36_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_sp29_36_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences341009
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6110.1791741566938116No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTAGT400.00259207934.5046231
CTAGTAC400.002599550234.4843833
TAGACTG450.00462842130.6527825
TATACCC500.007744509727.5875035
TGTATAT658.674871E-426.52255668
ACAGACC750.001988612623.00308447
GTATAGT750.001988612623.0030841
GCTTAAG800.002888118421.5717244
GTGTATG800.00289304321.565391
AGTAGTG850.00412513620.2849315
AGTACTG1701.0844815E-720.2849315
CTTGATA1108.237443E-418.812422
ACCATGC1301.1767243E-418.5794132
CTTAAGC950.0078041818.16565745
GTAAATC950.00784390118.1496733
ACAGTGT1351.5747466E-417.8860368
TGCGGCG1200.001475470317.2472510
CAGTACT1300.0025244515.9158684
GCATAGG1350.003233907515.33538832
TACTCTG3603.45608E-1115.32414468
Produced by FastQC (version 0.11.2)