FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_sp29_35_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_sp29_35_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences295641
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5360.18130096975723936No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGGG500.00771513327.6077933
ACATACT500.007734284727.5937719
AGATTAA500.00775987927.57510267
GTATTAT658.644653E-426.5369641
CGCCGTA658.687485E-426.5145267
CATAGAA953.0663438E-421.780872
GCCGTAT800.002909591421.54304968
TAAAGGT900.00572641419.1655854
ATAGAAC900.00572641419.1655853
AATACTG900.005732019419.162345
TCTAATA900.005737629719.1590982
ATCCTTA950.00783122118.1537976
GTACAAG1200.001473821417.2490271
TTCTATG1200.001477168217.2431892
TCCCTTA1503.4929535E-416.1127326
ATGCACA1300.002516711615.9221794
TGTGTGC1350.003240298215.3298738
AGGCATA1450.005187179414.2823136
GAACAGC1951.5950168E-414.1506526
AGTGTAG1750.001139088813.78755169
Produced by FastQC (version 0.11.2)