FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_sp29_34_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_sp29_34_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences433477
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8880.20485515955863862No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATAGGG605.425983E-428.7415852
TATAGTA500.007745096527.588745
TAGGCTA658.668991E-426.5276324
ATGAGGC700.001332986624.64133311
TGTAAGG750.001987737823.00654439
TCTACTC1202.4606798E-622.9906163
TTATATG1004.33736E-420.6939432
ATGTTAG850.004106580720.3022371
ATACGAC1056.0312456E-419.706243
GCATAGG900.005715545319.1743341
TGTTAGG900.005738471619.1610572
CCGAGCC950.00779315518.17145527
TCTAGAC950.0078418.152582
TATACTT950.00784521918.1504865
ATATAGA1200.001470821717.25691
TAATGGG3205.456968E-1217.25490816
GACCGAG1200.001477663217.2449517
AGCGAGC3551.8189894E-1216.5239234
ACTGTGC1907.0182377E-616.3373228
GAGTGTC1704.9618484E-516.2305437
Produced by FastQC (version 0.11.2)