FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_sp29_32_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_sp29_32_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences400167
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATGC553.2640997E-431.3458543
TCTATAA500.0077551427.5809042
TATAAAG658.682206E-426.5201022
GCTATAA700.001336737924.6288871
TTACACC700.001336737924.6288874
ATACGAT750.001997340522.9869613
ATGGACC800.002905710621.5502764
GGGTAGA800.002905710621.5502761
ACAATAC1004.3445977E-420.6882633
GTATCCC850.004117157320.2927579
GCTTTAC1258.760954E-519.3090461
GTGTAAC900.005746821419.15581
GTAACAC900.005746821419.15583
GTCCTGC950.00785138718.1476021
TTGCACC950.00785138718.1476024
TGTAACA1200.001481351717.2380662
TGTGTGG1452.7130544E-416.65822210
ATACACT1503.5331788E-416.0908724
GAGCGCA1350.003239552515.3323059
CTGACCT1400.0041401614.7773324
Produced by FastQC (version 0.11.2)