FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_sp29_31_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_sp29_31_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences334740
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACA501.8619456E-434.4894641
GTCTAAC450.00462496230.6572991
CTAGCCT605.4332754E-428.7326284
GGCATAG1155.5819328E-827.01598225
GTATAGT658.6596486E-426.5303541
TAACACA809.58127E-525.8593654
CTGAGGC700.001337735824.62428963
CATTTAG851.443305E-424.34551
TGCCCAA902.1199201E-423.0032910
GTGTTAG902.1263726E-422.9929731
GTAACAC750.001997094622.9861033
GTCTTAT1004.3348872E-420.6936781
TCTAACA1004.3480264E-420.68442
TATAGAA1004.3480264E-420.68442
CACTTAC850.004103651320.30309338
GTATTAT1056.023134E-419.7082651
ATAGGCT950.007803216618.16592634
GTGTGCG950.00783018418.1550649
TTATACT950.0078504618.1469254
TATTATG950.00785722818.1442132
Produced by FastQC (version 0.11.2)