FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_sp29_30_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_sp29_30_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435670
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGGG500.007758788327.57878955
AGCGAGC6350.021.74053634
TAGCCTA800.00289405221.56573114
TATCCAG6250.020.99120125
CTATAAT1004.3244718E-420.7031021
TTGGATA1004.3379038E-420.6935924
TATGCTT1004.3379038E-420.6935925
ATTATCC1004.3379038E-420.6935923
CGAGCAA6950.020.36024736
CCCTAAC850.00411256620.297161
GCGAGCA6900.020.00759535
GGGAATA6400.019.9551820
AATATCC7050.019.58865423
GCTCGCT6700.019.57684321
CCTTACA900.005742884719.1585332
ACAATAG1108.2426425E-418.8123575
TTGCTCG7000.018.73783719
AAGCGAG7200.018.69459533
AGCAAGT7400.018.65573138
ATATCAG6700.018.54648446
Produced by FastQC (version 0.11.2)