FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_sp29_2_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_sp29_2_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147159
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3680.2500696525526811No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCCGC150.002353845668.9823210
ACGGGCT200.00736368951.71916651
CGCTCTA450.00460017630.67966715
GGATAGA450.004615514530.6588121
CTAGACA450.004623199330.6483944
AGAGCTC500.00772302227.5929288
TAGAAGT756.152553E-527.5835534
ACAGTCT750.001987778822.9941088
TCAAAGC800.00289756821.5496523
ACCTGAA1004.3175602E-420.69469611
AGTGTGA900.00571963819.16175710
GCTGATC950.00781439918.1532448
AGAGGTG2251.095741E-716.85661769
GAGAGGT2402.2842869E-715.80307968
GAGGTGT1400.004124435614.77690369
CGAATAG5300.014.312222569
TAGAGAG2851.5938949E-613.30785666
ACGAATA6150.012.33410968
GACGAAT6600.012.01556367
AGAGAGG3401.1447124E-511.15511467
Produced by FastQC (version 0.11.2)