FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_sp29_29_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_sp29_29_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160152
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1780.11114441280783256No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTGGC350.001337857839.4673722
GTATGCA450.00460285530.6776479
GTACCGT450.004609904330.6680586
ACTAACC450.004616962330.6584723
TATACTT605.400192E-428.7513035
AAAGCGT500.007713649427.601256
GTTTTAG500.0077254327.5926251
CCATTGG953.0552087E-421.7836511
CTGCTCG800.002882654321.570229
TATCGTC850.004096438620.30138212
TTAATCC1206.3562875E-520.1196213
TCCTTAC2153.1341187E-919.2326469
TTGTCCT950.00780373518.1587164
CTTAATC1402.0727277E-417.245392
CTCCTTA3101.0732037E-915.561892569
TAATCCC1605.717752E-415.0944354
CCTTAAT1400.004117522414.7817631
ACTCCTT4102.7830538E-1013.44720969
GACTCCT4408.4946805E-1012.530354568
GGCTTAG2306.410055E-411.9967941
Produced by FastQC (version 0.11.2)