FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_sp29_28_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_sp29_28_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161022
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATAG150.002359516568.9434659
TAATTAA200.00733528951.7719348
GGCAGAT350.001341568339.4452834
CTGTTAG350.001347737239.408511
GTAATAC400.002592248134.493163
ATTTATA450.00462250330.651062
TGTAATA450.00462250330.651062
TCCGACG605.4080266E-428.74433
GTTAGTG500.00769953427.6116949
TTATCGA500.007746421327.57738559
AATTGTA750.001980732223.00974534
TAGAGTG750.001987913222.9954415
GTTTGGA850.00411716520.2837891
CTCACAT900.005699469219.1747916
TGTGTAG900.005740592719.15096369
TCTACTC2751.9463187E-1017.54924669
GTGTCAA1200.001465149317.2573099
TAGGCTT1906.957065E-616.3388655
AGTGGTG1350.003224451315.3350618
ATCTACT3654.3655746E-1115.11089669
Produced by FastQC (version 0.11.2)