FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_sp29_27_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_sp29_27_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173375
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATACC400.002598529734.4776459
CGCCGTA400.002598529734.4776467
CCTCGAT500.007708896427.60600711
AGCATAA658.630897E-426.53657349
CTTTTAT700.00132759524.648221
ACTGTAC953.0466105E-421.7942168
GTACTGA800.002885960321.567191
GTTCCGG800.002885960321.567191
CATGGGG850.004101119520.29853412
TCACACT850.00410798620.2926754
CACACTT850.00410798620.2926755
ATATTTA900.005708000619.170841
GAGGATA2608.731149E-1118.56488269
GGGAGAG950.007811520218.1566057
TGCTCGC1554.4792544E-415.58853720
CTGGATA1807.98767E-515.34110130
GCTCGCT1657.199854E-414.64377821
TTAGGCT2859.9831595E-814.52947810
AGGCTTA3152.1893356E-814.24119512
GGATAGT1500.00652736613.79105667
Produced by FastQC (version 0.11.2)