FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_sp29_26_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_sp29_26_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences328602
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGGGT451.0038462E-438.308383
AGCTATG450.00462232230.660717
GTATTGC605.4248644E-428.7400361
GGGTTAT605.4248644E-428.7400361
TATTGTA605.4345443E-428.7312852
AGGTTAC500.007734332227.5946397
AACCTAG500.007734332227.5946397
GTATTGT500.007740084527.5904351
TATAGAG500.007740084527.5904355
CTCTAGA750.001994022622.9920311
AATAGGA1202.462859E-622.9850272
TCTATAT850.004104130420.30254610
AGATACG850.00412227920.2870861
ATCTTTA1056.024474E-419.7074551
TGGATCG900.00573735719.1600235
CTACTTG950.00779727218.16820518
CGAGCAC950.007804129718.16543810
TAGGCAT1402.0654695E-417.26242623
TCTGGGG1250.001945085116.5492213
AGGCATA1503.488191E-416.11651224
Produced by FastQC (version 0.11.2)