FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_sp29_24_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_sp29_24_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences246839
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5450.22079169013000374No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGGC200.00734615451.7607964
CGCAGGA459.980527E-538.3413315
ATTGTAC553.245696E-431.370183
TAGATCC605.4034113E-428.7559995
GCTGTGC700.00132926224.6479998
CACTACT850.004106193320.2983517
CGATAAG900.0057083319.17455532
TTGTACT1150.001105315918.0037564
CGCAGAA1200.001470273717.253628
CCATGTT1300.002510684815.92639833
GGTGTAG1851.02314836E-414.90691669
TTATTCT1450.00519233114.278844
AGGTGTA2052.4471479E-413.45258368
CATAAAA1550.00808651713.3549182
CTTAGGC1950.002548733712.3871999
ACCTTAA2255.3157425E-412.27171419
TAGGTGT2306.460964E-411.99034667
TCTCCTG2100.004437726511.5023995
CAAAACT2450.001088150111.2676564
TTAGGCT2150.00528094711.2371816
Produced by FastQC (version 0.11.2)