FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_sp29_23_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_sp29_23_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences238816
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6480.2713386037786413No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGCG150.002349853769.02515412
AGGCGCT200.007323090551.8014524
TATATAG200.007365712451.725492
ATGCCCA400.002587598734.51257711
TACTGTG450.00461666430.664997
TTGCGCA500.007709109727.61006445
ACTAGGC500.007732807727.592713
AGAGCGC1253.4856494E-622.0787938
GTTATTC1004.3286654E-420.6945323
TAGAGCG1108.2136475E-418.8171547
GTGAGCC950.00780976418.1607079
GAATAGA1402.0847392E-417.2382181
TGTTATT1250.00194068516.5521562
GTTGTAG1250.001943405716.548691
GAAACGG1350.0032522815.31965460
ATACTCC6150.015.13282969
TAAGGTG1450.00516468714.29005424
ATGGAGC1450.00520075814.2750826
CGATACT8850.014.02137869
GAGCGCA1500.00650219413.8021389
Produced by FastQC (version 0.11.2)