FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_sp29_22_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_sp29_22_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences210170
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4250.20221725270019508No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGATC252.564133E-455.1921924
GTGTAGG306.304012E-445.9934921
GCACTAG306.304012E-445.9934921
GCGTAAA400.002587271434.51155511
TAGGGCG851.1969132E-732.4814630
GTGGGCG450.00460662630.6769359
TCATTAC500.007726127.5960961
AAGAGGT658.6165755E-426.5473487
GATGTAT658.640588E-426.5347081
GTAGGGC951.04273E-525.42956529
GGGCGCC1052.263441E-523.00770232
CTCTGTA902.11352E-423.0077029
GGTAGGG750.001983765323.00770228
TCTCCGC1103.2421507E-521.9618978
GGGAATA3200.021.56972120
CGGTAGG800.002886016921.56972127
AGTAGAG800.002894008521.559455
GAATATC3450.021.00703222
AGCGAGC3450.021.00703234
TATCCAG3450.021.00703225
Produced by FastQC (version 0.11.2)