FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_sp29_21_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_sp29_21_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131181
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3550.2706184584657839No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGTA200.00733878651.7603079
CTACACG200.007349873451.7405554
CTAGCCT400.00259030234.4937064
ACGCGAC450.004603423630.67277517
ACAGCTC500.0077028527.6054958
CACATAC700.001326012524.64776416
GAATACA700.001331927324.6289631
CTTACGT1253.4927252E-622.0591469
ACTGAAC1103.2430966E-521.9505393
CCTTACG1701.07735104E-720.27494268
AGAGAAC950.00778947418.161516
TCCTTAC2201.6097711E-615.66700167
TCCATCT1350.003218333215.3363878
TCTTCAC1708.974247E-414.21956626
AGGCTTA2304.2063664E-513.5026896
TAGGCTT2355.147986E-513.2153985
TTAGGCT2355.1664876E-513.2103564
ATCTTCA2153.586373E-412.84957525
CTCCTTA2801.9505587E-512.30978866
GGCTTAG2651.5827452E-411.7193157
Produced by FastQC (version 0.11.2)