FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_sp29_20_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_sp29_20_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166113
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3880.23357593927025577No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGAGT252.5637008E-455.187234
TCCTAGG200.00733722451.7692119
TATTGCA200.007354733551.738022
GTACTAT200.007354733551.738026
CGCGGAC200.007372274551.70687565
TCTTACT350.001346022539.419452
TAATAGG450.004603049730.6780531
CGTGTCA450.004609843730.66881210
CATTACT450.00461664630.659574
ACTATCT500.007713545627.6019298
TGTGGTC500.007713545627.60192911
CCTAATA500.007724927.5936152
TGCACAG658.6374546E-426.5323225
ATATTGT850.004096762320.3016511
TTGTTAT900.00571189419.16800710
TACTCTG3453.0013325E-1014.987569
CTCTGTG2454.93727E-614.06989968
GATCTAC6350.013.57135969
ATCTACT5450.013.28250169
TACTGTA2153.6318623E-412.8342395
Produced by FastQC (version 0.11.2)