FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_sp29_19_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_sp29_19_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126111
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2710.21489005717185652No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGAGCG150.002344666569.0444918
AATACAG200.007336732551.7628025
ATGTGGG350.001339006839.4539913
ATATGAT400.002589505934.4948433
GAACCGG450.004610620430.6620849
AGGTGCC500.007685008527.61779428
ATGAGTG500.00769992527.6068275
GGGAAAT850.004084891620.30721
AAAAGAT850.004094302720.2991396
GGATAGT1258.725599E-519.3017968
GATAGTG1301.1789689E-418.55941669
AGGATAG1554.5167416E-415.56596167
GCCCCAT1605.697029E-415.09748411
CTGCCCC1851.00775644E-414.9226099
TGCCCCA1750.001122939613.80341410
CCCCATG1750.001122939613.80341412
CCCATGA1800.001384797713.42531613
GCCTTCT2102.9461444E-413.1513291
GAGGATA1850.001722802213.04175166
CGAAGAG4408.385541E-1012.53363169
Produced by FastQC (version 0.11.2)