FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_sp29_18_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_sp29_18_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119680
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2740.22894385026737968No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGTTA200.00732276951.78610245
ATGTTAT200.00732276951.78610246
TTATATG200.00737139651.699544
CCAGAGT500.00768147227.61925532
CACTTAT500.00768147227.61925540
ATAACTT700.001318262224.67037425
CTGAACT950.00783853518.140194
TAGAGAG2109.893229E-716.41255269
TGCTCGC1400.00409451514.78983820
ATAGAGA2557.4361524E-613.51621968
AATAGAG2901.9375111E-613.07344767
AGAGAGG1900.002104859612.69813369
CGAATAG3451.0254207E-611.98829867
AGAAGTG2050.003708761711.773925
TTAGGCT2450.001091786811.2543214
GAATAGA3602.1552587E-510.53138766
TGCCGAC8253.6379788E-129.60880369
GACGAAT5059.461146E-79.55503169
ACGAATA4108.990072E-59.24707166
AGCCTAA3150.0086889338.7606846
Produced by FastQC (version 0.11.2)