FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_sp29_16_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_sp29_16_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448304
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12860.28685891716335343No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAAAGC700.00133339424.6402664
CGCCGTA2053.6379788E-1223.54798767
CTACAGC800.002894716821.5650461
CTTTACC1004.3354556E-420.6955152
TACAGCG850.00412149120.289722
GCCGTAT2553.6379788E-1220.28066868
GGTCGCC2402.7284841E-1120.11390764
CCGTATC2655.456968E-1219.5153669
AGGCATA1258.6955435E-519.32875424
TGGTCGC2707.2759576E-1219.15610163
GTATAAA1501.688443E-518.4021741
GGTTATC1702.4228102E-618.2668631
GGCATAG950.007806767718.1661225
TATGACT1653.8366208E-516.7255134
GTCCAAG1452.7153172E-416.6571391
TAGGCAT1503.5070043E-416.10729623
TTTATAT1554.5238988E-415.5772693
TTAGGCA1605.733462E-415.09890322
ATACTGA1605.7529326E-415.0921636
CAGTACT1605.7529326E-415.0921634
Produced by FastQC (version 0.11.2)