FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_sp29_15_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_sp29_15_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences227480
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6140.26991383857921575No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGG200.007346012651.7597429
GTGTTAA553.2536802E-431.355741
GCTGTAA450.00461569430.665698
CGCCGTA703.855751E-529.55098567
AGGAGCG500.00771499627.60519230
ACTGCCC500.007723286727.5991218
TGGTCGC1103.2694876E-521.93936763
AGAGCGC953.0558233E-421.7887788
GGTCGCC1154.6063044E-520.98548164
GTGGTCG1258.748143E-519.30664362
CCTTAAT950.00782699718.1533241
ACTGTCC1150.001106739617.9994268
GCCGTAT1150.001111652717.98755568
TCGGTGG1552.2465274E-517.79414259
CGGTGGT1402.084326E-417.23807560
GGTGGTC1452.7247248E-416.64365861
GTGGTGA1250.001934578116.5594719
TCGCCGT1250.001943133916.54855266
GGAGCAT1503.510452E-416.0994878
ATCTCGG2051.44208025E-515.1358756
Produced by FastQC (version 0.11.2)