FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_sp29_14_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_sp29_14_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92926
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2620.2819447732604438No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGGA150.00235089968.983852
CTCGATA150.00235089968.983851
GCAGCCG150.00235592768.9467266
GTACACA306.288631E-445.989231
TATGAAC350.001342979639.419343
GCATGGC400.002587082834.4919244
ATGCAGT2051.4249808E-515.134644569
GCAGTGT1550.00807828213.34452569
TATGCAG2509.218491E-512.41040968
GACGATA5652.5465852E-1111.59281169
GATATGC3702.3573248E-611.18054969
ATATGCA3105.4720324E-511.12043969
CGACGAT6452.8921932E-1010.154942568
CGATATG4301.41760975E-59.62047268
ACGATAT5008.543817E-68.96307467
CCGACGA7453.932655E-98.79186367
GCCGACG8202.183333E-87.98772966
TGCCGAC8453.7192876E-87.751405765
CTGCCGA9101.3720273E-77.19773464
GCTGCCG9553.1876152E-76.858574463
Produced by FastQC (version 0.11.2)