FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_sp29_13_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_sp29_13_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134240
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3280.2443384982121573No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATAACG150.002355641768.9657063
ACCACGC352.2637216E-549.279598
ACCATAG350.001344389439.423678
ATAATAC400.002594519434.4828573
TCCATTA450.004619519730.6514282
TTTATAC450.004619519730.6514283
CTACAAC605.4127554E-428.7357121
TGCATGG500.00772971627.5862852
CCATTAC500.00772971627.5862851
ATTTTAG658.6421706E-426.5252721
TACGTGT1151.6918475E-623.97913769
TAATACA750.001989655722.988574
CCTTACG2252.5283953E-1019.91600469
GTTTTGC1056.0051127E-419.7044891
TGATCAC900.00568810619.17858518
TATCAGC950.00777142318.16918628
TTACGTG1552.2388229E-517.79097268
CTTACGT1602.9409466E-517.23500467
GAAATGG1300.002508243515.92109833
TCCTTAC2959.291398E-915.19017268
Produced by FastQC (version 0.11.2)