FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_sp29_12_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_sp29_12_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences210516
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4990.2370366147941249No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTAAT350.001347021239.4183851
ACATATA459.975012E-538.3416633
GTCGTAA450.00462003430.6587451
GGCCGTC500.007748496727.57975853
CTTGGAC700.001334081224.6306343
AGACGTT750.001976908423.0214225
CCAGTAC750.001990633822.9940591
CCTGCCC800.00287606321.58257923
AGTGCAA850.00410401120.29852510
AGGTGAG1206.356403E-520.1245847
GAGGTGA1206.368047E-520.11986
GTTTGTA1056.011037E-419.7091921
TGACTCT950.00779325918.16615929
TGGACCA1150.001109818617.9910745
CCATGAA1351.5665955E-417.8927739
CCCATGA1250.001930710716.5635958
TGAAGCC1300.002508891715.92653512
CAGAACC1554.5192952E-415.5729184
CATGAAG1400.00410999614.78892510
TTCAGTA1400.00413607214.774872
Produced by FastQC (version 0.11.2)