FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_sp29_11_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_sp29_11_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147641
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4120.2790552759734762No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCAATA200.00732796751.78284512
CTCATGG450.004626094430.644521
GTTTTAC450.004626094430.644521
ACACAAT500.00771508627.5987826
GTTTGAT658.5892755E-426.55530433
TAAAACT658.6234097E-426.5372894
CTTCTAT658.657656E-426.5192951
TACAGTT700.001326057824.6501277
TAAAGCT750.0019853522.9989814
AGAGAGC800.002888328621.5615466
TTCTATT1004.3114452E-420.6990852
CACAATG900.00570149619.1723217
AACCGTG1400.00410011114.7900777
CTGAACA1550.00806894413.3542484
AAGTGGT1850.00170920413.0579057
AGGATAG2751.631948E-512.52790169
ATAATGG2509.168467E-512.42788215
TAATGGG2509.168467E-512.42788216
GGATAGT2255.3747534E-412.24950369
AGAAGTG2852.321897E-512.1047285
Produced by FastQC (version 0.11.2)