FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_sp29_10_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_sp29_10_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250238
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5310.21219798751588487No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGATA200.00739196151.67970758
CGGGGCC500.007684350527.62876728
GGACCAA750.001976670223.02397226
CGTCACC1154.568589E-521.0092615
TTACACT1056.0276024E-419.7032574
GTCACCT1258.6767286E-519.3285216
CTGAAGT900.00574066719.1559474
GGTGACT950.00780690318.1622688
ACGAAAT1300.00250691915.93009933
TGAAGGT1300.0025237215.9141694
AGAGAGG4150.015.77372569
TAGAGAG4800.014.35547468
TCGTCAC1450.005177696214.28501814
GTTCTGG1600.009984137512.9354371
TCTGAAC2000.003099325612.0682453
ATAGAGA6400.011.843266569
CAGAACT2550.001529836910.8174754
TCTTGAA2250.00746000810.7273292
GAATAGA8700.010.69235269
GAGAGGT3259.039002E-510.600965569
Produced by FastQC (version 0.11.2)