FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_qg2594_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_qg2594_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84
Sequences flagged as poor quality0
Sequence length75
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGATGATGTCAGATTGTTTGGATTCACAATCCAGAAAAAGGGAAGAGGATTACTGTAGATTGTCTAGAGTATCC11.1904761904761905No Hit
GTGAAATACAATGGGAACCAAACAAAATTTTTTAATTAAAATTTTCACGGAGACCTCCCGCGATAAAGTTCAAAT11.1904761904761905No Hit
CTACTACGTTCGAATCGTTTCGGATCGATGGATAGGAGCGGAGACTGTCCTCCCGATCTCATTCCGACACCTGAT11.1904761904761905No Hit
ATTCCGATGGAAGTGGAAGAATTTCCAGAAGGAGAGGAAGAGGTTCCACGGAAGAGATTAGAAGGATTTGTGTCT11.1904761904761905No Hit
GAATAGTATTGTCCAGCCCTTGCCATCCGTTGATACGGATTACGGTAGATGCGCTCTCTCGAATCCAGTGAGTTT11.1904761904761905No Hit
GGTGACCCCTAGTGACCCCTGGGGCCAAATGTTACCCCGAGGTACCAGAGGGAGACTCATTAGGACAAGTTAGCC11.1904761904761905No Hit
CAATGAACGGGCGATTGCGAATGGAGATCCAATTGAGGATAAGGCATGTTTCTGGGAGCTGTCTCTTATACACAT11.1904761904761905No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.1904761904761905No Hit
CTTCAAAGTCGAATGAATGTTGCCGCAAATCCAATCAAAGAAGCATTCTTCTTTCTGCACAGTTTCTGTGTTTCT11.1904761904761905No Hit
GTATTGTAGTATTTTGTAGGCCATATCTCGGTAGTCCGGGTTGAAATCACTGAACAGTGCCGAGAAGTCGGCGGC11.1904761904761905No Hit
ATTAACAACAAACGATGGAGGAAGAATGAAGTTCGGCGTCGGAGTATCTGATGGCCTTCGTTCCTGAAACTATTC11.1904761904761905No Hit
GTTTGTCACACTAATTTTATCACTTTTGAATCACAAGCAACACAATTACCAAAACCAGTACGATGATGATCCCTG11.1904761904761905No Hit
GTACAAACCAGATTCCGGAGTTGGTTTGATCTCCCAGCTCCCCAAAGCCTCCTTTACTTAAGCAACATCACATCT11.1904761904761905No Hit
GAACAACTGAAAAAGAACCTTGAAGACAAGGAAGAGCAGGTCCAAAGGTACTAATAGTCAAAATTCTTTACACCT11.1904761904761905No Hit
GGTCACCAGAGGTCACATGGGGTCACCAGAGGTCACTAGGGGTCCACAGAGGTCCCAAGGGGACACCCTGGCTCA11.1904761904761905No Hit
GTCCAGTTAGTACTTGGATCGGAGACGGCCTGGGAATCCTGGATGTTGTAAGCTTTTTGCATTTTGTTTTTTTCG11.1904761904761905No Hit
GGTGACTGGCTGCTTGTTCTTGGTGAACGTGGCAGCTATTCCAAAATTGCTTCTACTATACGGCTTAATGATTGA11.1904761904761905No Hit
AATCAGGATGGTTCGTTCACTGTCAAATACACAGCCCAGCGACCAGGAGCTTATCAAGTCAGTTTTTTTGTATTC11.1904761904761905No Hit
ACGTGACATCGGCTAATCATGAGACCTGAAATTCCAGTCCATCGCCCCTACTAAAAGTTCTCCGTTTCCCGCTTA11.1904761904761905No Hit
ATTTTAGAAATAATGGGGTGAATGTAGGGGATGTCAGGTACCAGAGAAAGGCGGTGCGAATAAAAGAAGCAGACG11.1904761904761905No Hit
GCAGAATCGTCGGAGAGTGATGTCGTCAGCATTGGAACTGAGTCTACAACTGCCATCGACGACTCACAGAATGAA11.1904761904761905No Hit
GACTAACCGTTCTTTTATTTCTAAACTTGTTGCTCACGAATCTAAACAGTAATTGTACTGTTTGTACAGTAGTCG11.1904761904761905No Hit
ATACTGGGGAAGTAGAGAGCGGTGATCATCATCTCATTTCCGAATAGAAGCACAAAACTGGCAAAAAATGAGATG11.1904761904761905No Hit
CTGTTCAACGAATGTGCAGACTTCCGTCGTACCGGTTCTGCGGCGCTGGATCTGGCTTACGTCGCTGCGGGTCGC11.1904761904761905No Hit
ATTATAAGCTCTAGAAATGACGATAACCACGACGAAGGATTCAGAGTTCCCACTTCTTCGGTAAGTTTCTTTAAA11.1904761904761905No Hit
GACTCTACTTTTGAAACTCGTGGTTTCTAGCTTGTTTCTAGTCTTTGAAACGCATTTTTTGACAATAAAATCAAG11.1904761904761905No Hit
CTTTTTGTCTCTTCTTTTCAGTTCAGAATGCAACTCCGGGGGACCAGCAGCCCCACACTCGTTTCAAAGCCCACC11.1904761904761905No Hit
GTCTATCCAGTGGAAAGTGATGTTGGTGGTGCTAAGCTTCTTCCAACAGATGAGTATGGACCTGTCTCTTATACA11.1904761904761905No Hit
TATCCATTGGAACAAGAAGTTATCAAGAAAAATAAAACACGAATACCAAATCAAAAAAGAATGCGGACCCTTCGA11.1904761904761905No Hit
CCATTGCGGATTGTGAACAGAACCTTCTGGCGAAAATACAAGGAACATGTGAAATCCGATCGAGATGCTCAAGTA11.1904761904761905No Hit
CTCATGGACAGTTGTTCGGATTCTTCACAAGCCAGTCGTTCTAAATAGAAAATTCCCAGAAAAATCTTTGGCTGC11.1904761904761905No Hit
ATACGGAACACTGTCGGAATCCCTATCCAGAGATGGCTCAAAAAGCATTGGGGGACTCTATGGGCTGTCTCTTAT11.1904761904761905No Hit
CTATTGCGATGAGAGACACGGTTATCAGATGAGTTTACATTAGATCTGTCTCTTATACACATCTGACGCTGCCGA11.1904761904761905No Hit
AAAGTAATCGGATGGTGCGGGTTCGGTGTCTGTTTGGTTCAATTTTTTTTCTTTTTTTTTAATATGTCATGGAAG11.1904761904761905No Hit
GAACAGAGGCGACGACGACACGGGCGGCGCTCGTCAATCCTTCGGGCCGCTGATAGGCTGTGGGTTATGCGCATG11.1904761904761905No Hit
TGCAAAAATCGGATGGCAAATGTTTACTGATGCAGTTATTCAAATAATTTCGAAGCAATCCGAAAAACCGATTGT11.1904761904761905No Hit
CTTAAAGACAAAGAGAGGGTGTCAGAAAAAGAACTTCCACAGCTCCAAATCACGACCAGTCAGAGCGTAAAGAAA11.1904761904761905No Hit
GAGTATATGAAGCAGATGGTACTATTCTGGAAAAGAAAGCATTTTCTTCTCAATTAGAGAGGCAAAGCCAAGTAG11.1904761904761905No Hit
ACAATCTTTTTCTCTTCTGCATTCAGTTGTTTCGTCCGCGTCGACTGTTCTGATCTTGTTGATTAATTGGAAAAC11.1904761904761905No Hit
CCTCTGGTGACCCCTAGTGACCTATGGTGACCCCTAGTGACCACGGGCGCCCGCTGATGCCCAACTGACGCGACC11.1904761904761905No Hit
AGCTAGTTTTATCCAGCCCTTCTGTCCCCTTCATTTTGATATTTTGGACCTTTGCTTGATTTTTCAAAAAACTAC11.1904761904761905No Hit
CCTCTGGTGACCCCTAGTGACCTCTTCTGACCCCTGTGACCTCTGGTGACCCCTAGTGCTGTCTCTTATACACAT11.1904761904761905No Hit
ACCAGGGTGTTCGTCGTGGTGCCCGTCTCTCGAACAACGGACCGATGCTCGGACGGCGAGTGAAACAGTCGGATG11.1904761904761905No Hit
TAATTGATGTTTACAGGTGAAATTCTCTGGAATTCAGACATTGTTTTGAGAACATATATCCCAAATTTTGATGAT11.1904761904761905No Hit
CCTTTTCTGAATCCCATCATCTAACTCTCTGTTTCTGAAAGTTCACATAGTAGTCTAGCTTCCAAAGATAGTGTC11.1904761904761905No Hit
CTCAATCCCCTTGATCCCTTAGTATATACACTCAAAGTGTAATGATAATGATGACTTTATTATTACCTGTCTCTT11.1904761904761905No Hit
ATTTGACCCTTGTTGTTGTACTGGCTGCGAGCTTGATTGTCCTCGGGAGCTGCTATTTGAGGGTCTGTGAGAATG11.1904761904761905No Hit
AGCTTAATAGCCGGTGGAAGGTTAGCAGTCTGCACCCTCTTCTTAATGTCTCCAATTGAGATCTTCAGTCTAGCT11.1904761904761905No Hit
CCTCCGAAGGGCAAAGTTACGGTATGTTCTTTGCCCTGGCGGCTAACGACCGTGCAGCTTTCGATAATATTCTCG11.1904761904761905No Hit
GTATATTCAGTTAATCGACAAATGGATAAGAGAAAAAAGAGATATTGGAACGGCGGCTGTATTCGGGATTCATAG11.1904761904761905No Hit
CAAAATGAGGATGCCAACATTTTTTGGAAATGAAACTCCGACAAGCCTCCCCACCAACTTTTTATCAGTGGCGTC11.1904761904761905No Hit
GTGTATGTTTGGGTGGTAGGCTCTGGAAAATTATTTTTTGAAACTAAAAACCAGGGGTTTTAGAACCTAATTGAC11.1904761904761905No Hit
CATATACACAGTGCACAAAGCACACCACGCATACAGCACGAAACGCACCGTCCGACGAGACACACACCGCATCCT11.1904761904761905No Hit
GGAGAAAGAGAAACGGACCGACTGCCATCGTTAAGAACAAAAAGGGCGCGAGGGCGCGGGGTGACGACTCAAACA11.1904761904761905No Hit
CTTGGATGCATTTCAAGAAATTATCTGGTGGTTTGGAGTAACATTACCATAGATAACTACATTCATGTTTCTCGC11.1904761904761905No Hit
CAATAGAATTCCTGTCGTATTCTCTTGGTAGGGAATTCTCATCGGATTCCGTCTCCATTTGACATTCGACTGCCA11.1904761904761905No Hit
ACCGAACATGAGTGAGCCGTTCTGCAGAAGACACCGGCCGAAGAGGACACGTGGCAAAAACACGAAAGGCTGTTG11.1904761904761905No Hit
GTATTGGTCCCCCCAGTTCACATTTCAACCTTCTAGAAAAACCGTATCAGTAAATATTCCATCTTCATGTTTACC11.1904761904761905No Hit
ATAACAAAAAAAATCAAACCACTCCCTACAAAGACAACCTAACATCAAATATAGAATAATCTCAAAAACACAACG11.1904761904761905No Hit
TCATTCCCTCATTTCAAGCAAATGAGTCACAAAACTTGCAAAACACAGACACAATTACATATATTTTAAGTACTC11.1904761904761905No Hit
CCGTTGATCGACGAGGATTATGTGCCCGTTTATTCGAGGGTAAACTTTTAATTCGGAAAGCGATTCCAAAGAAAA11.1904761904761905No Hit
CAAAAACTCACAATCACTATCATACTCCTACTCAATCACAACACTAATTCAATCAAAATTAACTAAACTCAAATA11.1904761904761905No Hit
GAGGACATGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAAGGAAATCTAAAGAAAAAGATTGACTG11.1904761904761905No Hit
AGTCTAGCTGGGTCCATTTCTTTTTTCCTTTTTAGGCTGTCTCTTATACACATCTGACGCTGCCGACGACTCCTT11.1904761904761905No Hit
CGACCAGTCCGTGTGTGGAACGAGTAATTGGCTCTGAAGAAAAAGAAAAGAGAGAAATGAAAGAAAAATGGAAAA11.1904761904761905No Hit
TCTGAATTTCGAACAAGAAGAAAAAAGATTTCGAAACGTATGTAATGAAAAAGAGAGAAAGTTTAAATTCGTATT11.1904761904761905No Hit
CAACTGATGCAAGTGGTAAACCATTGTCTCAAGATGGATCTCCACTTCCAACAGATAACAATGGAAACTATATTC11.1904761904761905No Hit
TCGCCTCTACCTCATATACATTTAGCTTCTTTTCAGCCGCAACACTTCGAATTCTATCTTACTCAATACTCATTC11.1904761904761905No Hit
CAGTAGACCTATCATAGGCGTATGATAGTATGCTGGCGGTGCCCCTCTTTGACCGACGTGCGCCTCATTTCAGAG11.1904761904761905No Hit
TTTCTACACTGCTTGCTTCACTGGCTGAGTATCATCTTTTTTCAAGCAGTTTTTTGAGAAGCCAAAAAATTATTT11.1904761904761905No Hit
GTTTTGTATGGCCTGGTTCTATTGGAAGACAACTGTTTGAATACCTTGATAAGAACCCAGCAGTTTCCGCCGACT11.1904761904761905No Hit
CCCTCTCGCCGCTTTTTCATTCAACATTTCCACAAAAATGTCGACTTCGGATGCTGATTCAACTGTCTGCATCAT11.1904761904761905No Hit
ACTGTAATTGGCATACGTCTTCATTAATTTTACGAATTTTACGGATTTTACGGATTTTACGAGGGTTGATTTCGT11.1904761904761905No Hit
ATATTATTAAAAAAAAATTAACATAGAAACTTATCAACTGAACATAATAAACACTATCTTATACAAAACTAACAA11.1904761904761905No Hit
GTAGTACAAGACACTGCAAAATAAGAGGAAAAGACAAGGAGAAGAGCGCGAATATAAACCTGTCTCTTATACACA11.1904761904761905No Hit
ATTCCAATCTTCCGGTCACTAATCGTTCTATTAAATCAGTGGGCGGAGTCTTTCAACCAACACATAAATGACCTT11.1904761904761905No Hit
ATATGAAGGAAATAGGTAGCAGAGAAAACGAAAACGAAGTGAGAGAGAGACAGGGCGCTAAATAAGGAAGAGGAT11.1904761904761905No Hit
GAGTGAATTTCAGAAAGAGAACGAATTCAAGATTTATTTTTTTCAGGGAGAATTGGTGAAATCATTGGCAATTAC11.1904761904761905No Hit
GAAGAAAAGGTTCGCAAAGACAGAAGTCCTACCCCAGAAGACGTGATCGCTGTCTCTTATACACATCTGACGCTG11.1904761904761905No Hit
TTATAACTCTTTCAAAAGTGGCCGGATCTCAAAATCTCAAAGTGTATGTGGAAACTATCTATGCCATTAGGGAAC11.1904761904761905No Hit
AATAAAAGGAGTGATTCGGAACCAGAAAAAATCTGAAAAAAAAATTCTAATAGCTAGAGAGTCTCAAGAAATGGT11.1904761904761905No Hit
CTAATATGCTGCGCGCTTTTTGACGTTTTTTACTTTTTTAAATGAAAATTACCGGCAGTATTCTTCCGACAGTTG11.1904761904761905No Hit
GGCGTGGTATTGCGCTAACGCGTGATGACTGTATTCGCCGCGATGTGATTAAGTCTGTCTCTTATACACATCTGA11.1904761904761905No Hit
AATATAGACAGTCGAAAGAACCAACAGTTCAACTGATATTTGAACTAAAAATTGGCTCCACGTTGAGAGCTGTCT11.1904761904761905No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTGTA50.069.066
CAGCCCA50.069.032
CGTTCAC50.069.014
ACAGCCC50.069.031
AGCCCAG50.069.033
TTCACTG50.069.016
GGATGGT50.069.06
ACTGTCA50.069.019
TGGTTCG50.069.09
CAGTTTT50.069.060
TCAAGTC50.069.054
CCCAGCG50.069.035
GCCCAGC50.069.034
AGCTTAT50.069.048
TTCGTTC50.069.012
TTGTATT50.069.068
CCAGCGA50.069.036
GCGACCA50.069.039
AGGATGG50.069.05
AATCAGG50.069.01