FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_qg2592_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_qg2592_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173156
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3520.20328489916606993No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTTAA400.002586328234.510739
CCCTTAT501.8515254E-434.51071
GCCGTAT553.2673724E-431.32806668
GTACTGA450.00460495830.6761786
ATAAGAC450.004611479630.667313
TGAGCCT450.004611479630.667314
TGTACTG605.4027536E-428.7506035
GGTCGCC605.4394413E-428.71739464
CGCCGTA605.4394413E-428.71739467
ACGTTTG500.00770538627.60855917
GGTTTAC500.00770538627.60855947
TATTACG500.007727169427.5926022
TAGGCAT1052.2558288E-523.01378829
ATAACGT902.1159207E-423.0004844
TAACGTT953.050643E-421.7899325
GTGGTCG800.002908694321.53804662
TATATAA1004.3221467E-420.6944522
AAGTGAG850.004098920.3004136
GTCGCCG850.004133358620.27110165
CCGTATC850.004133358620.27110169
Produced by FastQC (version 0.11.2)