FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_qg2591_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_qg2591_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences318641
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6260.19645933825213954No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAA200.00734027951.7747516
AACCCCG500.007732326627.5958527
GGCATAG906.860055E-626.84616931
TACCCTC658.658818E-426.5303085
CATATGG700.00133167724.6430261
GCCTATT700.001336561724.62755260
CGCCGTA1151.7027232E-623.98132967
TTAGGCA902.1125488E-423.01461828
TTAAGCT750.001993435722.9929354
TCGCCGT1253.5137218E-622.0628266
GCCGTAT1253.5137218E-622.0628268
TATACTG800.002900047221.5558745
GGTCGCC1304.9298596E-621.21758364
CTGTGTC1004.3251275E-420.7001429
TATATAC1356.815975E-620.4381623
GTATATA1601.3554163E-619.4063821
GAGTAAT900.005725277619.1667981
TAATACC900.005735681419.1607784
CTAAAGG900.005735681419.1607783
AGGCATA1651.8088667E-618.83014330
Produced by FastQC (version 0.11.2)