FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_qg2586_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_qg2586_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176136
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2910.16521324431121406No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG451.8656028E-645.952481
CTATTAC405.0152055E-543.080451
CTACACA400.002599929734.4741484
CGCCGTA450.00464200230.62628767
GTGGTCG450.00464200230.62628762
GAGCCAC500.007702827527.61069531
ATTATGC500.00772424427.5949956
GTGGTCA500.00772424427.5949956
TTACATT500.00774570827.5793174
GTATACA500.007756457727.5714861
CTATTGA700.001337398824.6173971
TCTTACT851.4466455E-424.3277822
CTAGAAG750.001998339522.9762363
TAAAAGT1103.26553E-521.9380954
GATATCT850.004097335520.30198111
GATCCTT950.00781695618.1546026
TGAGCAG950.00782978418.1494455
GTAGATC950.0078426318.14428952
TTAAAAC950.00785549318.1391353
GTCGCCG950.00786837318.13398465
Produced by FastQC (version 0.11.2)