FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_qg2576_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_qg2576_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179657
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3190.1775605737599982No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGTAC553.2494072E-431.3583183
GGAGGTC450.004609955530.6700137
GTGTTAA450.004616243830.6614691
ACTAGAC450.004616243830.6614693
GGCATAA500.007703238627.610743
CTTAAGA500.007745246427.57996263
GTAATGA750.00198525723.00250633
CATATGT800.002892862521.5588444
AGGCATA850.00410427720.2963342
ACATTGG900.0057215919.1634181
TGGGGTC950.007804492518.15987215
TAGGCAT950.007804492518.15987241
GTATTGA950.00781704318.1548161
TGTATCC950.00781704318.1548163
ACCCCTA1600.00997050212.93530710
ATTTGGG1600.00997050212.93530712
TAAGCCT6000.012.6478564
TACTGTA2204.420491E-412.5398362
CTCCTTA5800.012.4823169
AAGCCTA6150.012.3393715
Produced by FastQC (version 0.11.2)