FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_qg2572_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_qg2572_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108910
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1230.1129372876687173No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTTGG200.007337757451.75620723
TAGTATA200.007351108451.732432
GTATTAA350.001341660539.43331
TTATAGT350.00134469739.4151844
GTATATA601.3303452E-534.504141
ACAGGGG400.002590375334.4882854
TATACAA400.002590375334.4882855
GGCATAG553.226839E-431.38181925
GTCACAG500.007700178327.603311
GCATAGG658.5774955E-426.55384426
TAGGCAT1004.283357E-420.71229
TCCCTTC850.00410471120.2872288
CAGAATC850.004115652320.27791260
AAGTGGG900.005713030719.160167
GGTCACA950.00776412818.1684228
AGGCATA1150.001097186518.01043330
TAGGCTT5300.014.3158935
TACTCTG2253.4425386E-513.788980569
TTAGGCT5100.013.5248174
AGGCTTA5250.013.1383956
Produced by FastQC (version 0.11.2)