FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_qg2567_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_qg2567_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96726
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1930.19953270061824122No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTGC150.002348101269.006734
ATTACCA200.0073359451.75504335
CAAGACA200.0073359451.7550434
CCCTGTG200.0073359451.7550439
TACAGCG200.007350967751.72828367
ATAATCC306.279646E-446.0044863
TAATCAC400.002583400634.5033659
AACTCTT652.2982158E-531.8492575
CAACTCT553.2333276E-431.3666934
TCTTAAA450.004599794730.6696552
TATCTCG450.004599794730.66965532
GTCACAA605.3829345E-428.75280429
ATTGCAT500.007696832527.6026883
GGTCACA851.4290493E-424.35531628
TCGTACA1304.8411985E-621.23283821
AGGGGTA1258.629476E-519.32188232
AGACACA900.0056940919.1685356
ATCGTAC1301.16601055E-418.57873320
CGTACAA950.007779626618.15966622
ACATACG1602.9029632E-517.25168211
Produced by FastQC (version 0.11.2)