FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_qg2562_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_qg2562_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences370576
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6210.16757696127110228No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACACT751.6650592E-632.2003174
GAGCGCG500.00771260227.6114659
GCCACTG500.00778411327.55930565
CGCCGTA1254.1982275E-927.55930367
GGTCGCC1457.1122486E-1026.13382364
TGGTCGC1501.0550139E-925.26269563
ACAGACG851.4366633E-424.3630588
GCCGTAT1452.0343577E-823.76122968
ACCATGT750.001985681723.0095548
CCAGTAG750.001991935522.9971183
TCTATAC750.001991935522.9971183
GTGGTCG1605.7732905E-821.53361162
CTCGGTG1657.9924575E-820.88107758
TCGCCGT1658.003917E-820.8782666
GTGTACA1004.338724E-420.6918141
GTCGCCG1851.1899829E-820.48326765
CTAGAGT1056.0120126E-419.714484
GTACTCA1056.0120126E-419.714484
GTATTAG1056.0284534E-419.7064911
GTCTACA1056.0284534E-419.7064911
Produced by FastQC (version 0.11.2)