FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_qg2559_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_qg2559_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144110
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4760.3303032405801124No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG150.00235013169.009037
ATATGCC200.007332141551.7747637
GCATTAT400.002582756334.516511
TATGGGT400.00259157934.492534
TTATACT501.8557216E-434.492534
ATTGAAC553.2339062E-431.3786478
ATGGGTG450.00460645330.6706835
TGAAAGG450.00461429330.6600283
ATTATAC450.00461429330.6600283
GTGTAGA1601.8189894E-1227.9960169
ACATAGA500.00769482627.6132091
TAGATTG500.007707895727.6036155
TACACTT500.007707895727.6036155
TCCAGTA500.00772098227.5940272
GGTGTAG2150.027.24477669
TTGAAAG800.00289089221.5578332
CTAAGCC850.004108124420.2897243
AGGTGTA2900.020.19871368
TTAGGTG5100.019.59298569
ATTCTAG900.005694811719.1758381
Produced by FastQC (version 0.11.2)