FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_qg2556_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_qg2556_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119406
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1640.137346532000067No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACTTG200.007338206351.75848843
AGGCAGC200.007350381551.7368056
TAAGCCA350.001339125339.45157634
TAGACAG400.002590299834.4912035
TCCGAAG450.004602581330.67169637
TTAGGCA605.3751055E-428.76677328
GTATAAT1103.614514E-828.2200741
GGCATAG500.007685703727.616131
TAGGCAT800.002874642121.57507929
AGGCATA850.004085093320.30595630
TTAAGGT850.004104988620.2889424
TATAATC850.004104988620.2889422
CCTGTGA1200.00146881717.2456023
CCCTGTG1200.00146881717.2456022
AATGAAC1250.001919405716.5696636
ATACTCC2051.4304524E-515.13613768
TGGGGTC1400.004109032514.7819445
CTGTGAC1450.005187502614.27222254
CGATACT2901.2374585E-714.26624568
AGTGACC4455.456968E-1213.9514986
Produced by FastQC (version 0.11.2)