FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_qg2555_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_qg2555_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144679
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3690.2550473807532537No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCACG252.561498E-455.191977
CTATAGC200.00733021551.77830537
CGTACAC200.007360382551.7245753
ACGGAGG200.007380545651.68881662
TGTTAAG200.007380545651.68881659
ACACCGT459.980819E-538.327768
GTATTGA807.030394E-834.4949841
CACCGTA400.002590708734.4949849
AACACCG400.002590708734.4949847
GGTAAAC500.007692355727.61509733
GTATATA500.00771840427.5959851
GCCGTAT658.680153E-426.50708668
CGCCGTA700.001337408424.61372467
GTATAAA902.1159613E-422.9966561
TATTGAG750.001990333422.9886992
CTAGACA900.00572694419.1572514
CTATTCT950.00782436618.1489734
TCGCCGT950.00785565118.13642766
GAATAAG1300.00250986215.9207611
TAAAACA1554.5069508E-415.572994
Produced by FastQC (version 0.11.2)