FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_qg2554_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_qg2554_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128886
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3330.2583678599692752No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTACC200.00732237651.78907841
TCCCACC200.007333645651.76894813
CGTTAAT200.007356222751.7287371
TACTGAG350.001343515739.4276857
GTGTCGT400.00258316834.5126311
ATCCTGA501.8525182E-434.4992266
TATACTG450.0046081230.6659775
TCAACCT450.00463447230.63027457
ATACTGA605.3965393E-428.7493536
CGAAGTT500.007681474627.6208418
TAGAATA756.122681E-527.5993794
TCCTATA500.007710688727.5993792
TTATACT700.001327599724.6423024
CTGAGCA953.0376684E-421.7974519
AGAGTGT800.002885993621.5620166
ACCCCTA1055.9528527E-419.7291720
GACCCCT1301.1639497E-418.5909519
TGACCCC1855.3331514E-616.79645718
CCCTAGT1452.699382E-416.6547972
CCTAGTG1250.001928314816.5596283
Produced by FastQC (version 0.11.2)