FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_qg2547_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_qg2547_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146863
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3160.2151665157323492No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCGAT200.00732649951.78532419
GGCGTGT200.007395983751.661954
GTGTTAA405.0007046E-543.0956041
ATCGCTC350.001339474639.4554868
CAACACG400.00258032934.5235528
TCTATAC601.3345949E-534.53
TAGTACA450.004601991730.6771284
CCAGATT500.007687628327.6188413
CGAGGAG658.586646E-426.55657817
CTATACT809.4841205E-525.883834
GTATAAT750.001992679222.9843221
ATATAAT1004.3299017E-420.685891
TTCTATA850.004119578720.2802852
GTATTTA850.004119578720.2802851
ATAAATG1150.001103954918.0000023
CCTAGTG1400.004108026614.7857143
CTATTTG4557.2759576E-1213.6390491
ACCTCTG2406.2534375E-512.94633210
CCCTAGT1600.00999285712.9286812
CTCTGTG2153.6727954E-412.81535569
Produced by FastQC (version 0.11.2)