FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_qg2544_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_qg2544_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140722
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3280.23308366850954362No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATA150.002347059569.030951
GATCCTA200.007342985451.75480311
GCGGATC200.00736366751.71801856
AGTTGTG400.002582934534.51547636
AAGAGCG400.002587446734.50327
GAACGTG450.004606956630.6695129
TAAATAG450.004614982330.6586072
GTATAAA700.001324568424.653911
ATTATAT851.4370892E-424.3465423
TAGAGGT750.001987423522.9939564
TTATACT900.00571863419.161634
GGATAGT1503.5142308E-416.0900569
GAATAAA2051.4189E-515.1531351
CCTAGTG1750.001128424413.7963733
AGTGACC2557.3765423E-613.5306676
CCCTAGT1800.001395725113.4131412
TAGTGAC2609.013613E-613.2704615
CCCCTAG1850.001706547813.0599091
AGGATAG2153.6375923E-412.82927668
CTAGAGA1900.0020967912.7071884
Produced by FastQC (version 0.11.2)