FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_qg2542_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_qg2542_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135269
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2730.20182007703169239No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGAG350.001346372339.4120862
AGCACAC450.004609509430.66518610
GGTCAAG450.004609509430.6651861
TAATTCC450.004609509430.6651864
AGTGCAC500.00771300527.5986678
GGTATAC500.00771300527.5986678
AGGCATA700.001325280224.65078724
GTTCTAT700.001328143424.6416661
TAGAGTG800.002887166621.5614595
TAATATT1055.987305E-419.7133354
TAGGACA1055.987305E-419.7133354
CGTACAG900.005698210619.17283422
AGTTAAG1108.188144E-418.81727427
TAGAACT950.007801838718.1570194
TTCTACA950.0078185118.1503032
ATCGTAC1402.0604208E-417.2555520
GTACATA1402.0662625E-417.2491669
GTACAGT1250.001924549816.56532923
TCGTACA1503.4878743E-416.1051821
GTTAAGG1300.002507107315.92230928
Produced by FastQC (version 0.11.2)