FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_qg2525_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_qg2525_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157481
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3010.19113416856636672No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG404.9778057E-543.1315575
CACTGAC350.001343273139.4345667
AGAGTCG350.001343273139.4345668
ACCTTGT400.002587626234.5052456
CTGGCTG400.002591664734.494284
TTATACT553.2475224E-431.3584354
AGGGAGG450.00461444330.6615813
TTAGGCA500.00770925227.60419722
GTGAAGT800.002885894421.56577932
ACAATGC850.00410103320.2972038
TCTACAA850.00410859220.2907542
TAAGAGA900.00571838819.1634884
AGGTGTA1301.1771822E-418.56794468
CTCCACC950.00778408318.16642850
AAGAGTC950.00779837518.1606547
GAGAGCT1200.001467627517.2526237
GGTGTAG1402.0758845E-417.24166369
TTAGGTG1901.2757684E-414.51929469
TAGGTGT1709.087381E-414.19901667
CTTAGGT2001.9724919E-413.79332968
Produced by FastQC (version 0.11.2)