FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_qg2523_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_qg2523_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148272
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3290.22188950037768423No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAAG200.00732327951.7913349
TAGTACG200.007362531551.721395
TTAAAGT450.00462232330.649714
TTGAACT1300.002517095315.9142744
CTTGTGT1850.001733394113.03363569
CTCCTTG2952.3737957E-612.84428569
CCTTGTG1950.002574498812.36524468
CGATACT6600.012.00392269
CCCCTAG2601.3244215E-411.9478061
TAGTGAC2953.3223747E-511.688455
AGTGTGT3359.634428E-611.3297458
GTAAGTG3451.3454444E-510.9939175
ATACTCC4703.0338924E-810.99341669
TAAGTGT3156.4411E-510.9537216
ACTCCTT3156.552791E-510.93524968
CCTAGTG2550.00152338410.8175443
CCTCTAT3853.806159E-610.7545629
ACGATAC7450.010.63434768
TTGACCT5201.155604E-810.6095165
CTATTTG4251.072256E-610.5577991
Produced by FastQC (version 0.11.2)